Toward a script theory of guidance in computer-supported collaborative learning

This article presents an outline of a script theory of guidance for computer-supported collaborative learning (CSCL). With its four types of components of internal and external scripts (play, scene, role, and scriptlet) and seven principles, this theory addresses the question how CSCL practices are shaped by dynamically re-configured internal collaboration scripts of the participating learners. Furthermore, it explains how internal collaboration scripts develop through participation in CSCL practices. It emphasizes the importance of active application of subject matter knowledge in CSCL practices, and it prioritizes transactive over non-transactive forms of knowledge application in order to facilitate learning. Further, the theory explains how external collaboration scripts modify CSCL practices and how they influence the development of internal collaboration scripts. The principles specify an optimal scaffolding level for external collaboration scripts and allow for the formulation of hypotheses about the fading of external collaboration scripts. Finally, the article points towards conceptual challenges and future research questions

Purification and proteomics of influenza virions

This chapter describes a basic workflow for analyzing the protein composition of influenza virions. In order to obtain suitable material, the chapter describes how to concentrate influenza virions from the growth media of infected cells and to purify them by ultracentrifugation through a density gradient. This approach is also suitable for purifying influenza virions from the allantoic fluid of embryonated chicken eggs. As a small quantity of microvesicles are co-purified with virions, optional steps are included to increase the stringency of purification by enriching material with viral receptor binding and cleaving activity. Material purified in this way can be used for a variety of downstream applications, including proteomics. As a detailed example of this, the chapter also describes a standard workflow for analyzing the protein composition of concentrated virions by liquid chromatography and tandem mass spectrometry

Detection of very-high-energy gamma-ray emission from the vicinity of PSR B1706-44 with H.E.S.S

The energetic pulsar PSR B1706-44 and the adjacent supernova remnant (SNR) candidate G 343.1-2.3 were observed by H.E.S.S. during a dedicated observational campaign in 2007. A new source of very-high-energy (VHE; E > 100 GeV) gamma-ray emission, HESS J1708-443, was discovered with its centroid at RA(J2000) = 17h08m10s and Dec(J2000) = -44d21', with a statistical error of 3 arcmin on each axis. The VHE gamma-ray source is significantly more extended than the H.E.S.S. point-spread function, with an intrinsic Gaussian width of 0.29 +/- 0.04 deg. Its energy spectrum can be described by a power law with a photon index Gamma = 2.0 +/- 0.1 (stat) +/- 0.2 (sys). The integral flux measured between 1-10 TeV is ~17% of the Crab Nebula flux in the same energy range. The possible associations with PSR B1706-44 and SNR G343.1-2.3 are discussed.Comment: 4+ pages, 2 figures; v1 submitted to ICRC Proceedings on 15 May 2009; v2 has additional references and minor change

The H.E.S.S. central data acquisition system

The High Energy Stereoscopic System (H.E.S.S.) is a system of Imaging Atmospheric Cherenkov Telescopes (IACTs) located in the Khomas Highland in Namibia. It measures cosmic gamma rays of very high energies (VHE; >100 GeV) using the Earth's atmosphere as a calorimeter. The H.E.S.S. Array entered Phase II in September 2012 with the inauguration of a fifth telescope that is larger and more complex than the other four. This paper will give an overview of the current H.E.S.S. central data acquisition (DAQ) system with particular emphasis on the upgrades made to integrate the fifth telescope into the array. At first, the various requirements for the central DAQ are discussed then the general design principles employed to fulfil these requirements are described. Finally, the performance, stability and reliability of the H.E.S.S. central DAQ are presented. One of the major accomplishments is that less than 0.8% of observation time has been lost due to central DAQ problems since 2009.Comment: 17 pages, 8 figures, published in Astroparticle Physic

Role of a Transbilayer pH Gradient in the Membrane Fusion Activity of the Influenza Virus Hemagglutinin: Use of the R18 Assay to Monitor Membrane Merging

It had been suggested that influenza virus-mediated membrane fusion might be dependent on a pH gradient across a target membrane. We have designed experiments in which this issue could be addressed. Two populations of liposomes were prepared, both simulating the plasma membrane of target cells, but with the pH of the internal aqueous medium buffered either at pH 7.4 (physiological cytosol pH) or at pH 5.0 (endosomal pH at which influenza virus displays maximal fusion activity). By monitoring fusion using the R18 assay, we found that the internal pH of the target liposomes did not influence membrane merging as mediated by the influenza virus hemagglutinin, thus demonstrating that a transmembrane pH gradient is not required in this fusion process

Spatially resolved X-ray spectroscopy and modeling of the nonthermal emission of the PWN in G0.9+0.1

We performed a spatially resolved spectral X-ray study of the pulsar wind nebula (PWN) in the supernova remnant G0.9+0.1. Furthermore we modeled its nonthermal emission in the X-ray and very high energy (VHE, E > 100 GeV) gamma-ray regime. Using Chandra ACIS-S3 data, we investigated the east-west dependence of the spectral properties of G0.9+0.1 by calculating hardness ratios. We analyzed the EPIC-MOS and EPIC-pn data of two on-axis observations of the XMM-Newton telescope and extracted spectra of four annulus-shaped regions, centered on the region of brightest emission of the source. A radially symmetric leptonic model was applied in order to reproduce the observed X-ray emission of the inner part of the PWN. Using the optimized model parameter values obtained from the X-ray analysis, we then compared the modeled inverse Compton (IC) radiation with the published H.E.S.S. gamma-ray data. The spectral index within the four annuli increases with growing distance to the pulsar, whereas the surface brightness drops. With the adopted model we are able to reproduce the characteristics of the X-ray spectra. The model results for the VHE gamma radiation, however, strongly deviate from the H.E.S.S. data.Comment: 8 pages, 7 figures, accepted for publication in Astronomy & Astrophysic

Adaptive auditory risk assessment in the dogbane tiger moth when pursued by bats

Moths and butterflies flying in search of mates risk detection by numerous aerial predators; under the cover of night, the greatest threat will often be from insectivorous bats. During such encounters, the toxic dogbane tiger moth, Cycnia tenera uses the received intensity, duration and emission pattern of the bat's echolocation calls to determine when, and how many, defensive ultrasonic clicks to produce in return. These clicks, which constitute an acoustic startle response, act as warning signals against bats in flight. Using an integrated test of stimulus generalization and dishabituation, here we show that C. tenera is able to discriminate between the echolocation calls characteristic of a bat that has only just detected it versus those of a bat actively in pursuit of it. We also show that C. tenera habituates more profoundly to the former stimulus train (‘early attack’) than to the latter (‘late attack’), even though it was initially equally responsive to both stimuli. Matched sensory and behavioural data indicate that reduced responsiveness reflects habituation and is not merely attributable to sensory adaptation or motor fatigue. In search of mates in the face of bats, C. tenera's ability to discriminate between attacking bats representing different levels of risk, and to habituate less so to those most dangerous, should function as an adaptive cost–benefit trade-off mechanism in nature